Ji Suk Chang, Ph.D.Assistant Professor - Research
Gene Regulation and Metabolism
EDUCATIONPh.D. Case Western Reserve University, OH, 2005, Molecular Biology
M.S. Case Western Reserve University, OH, 1999, Biochemistry
B.S. Seoul Women's University, Korea, 1995, Chemistry
RESEARCH INTERESTSObesity is a rapidly growing global health issue that is often associated with metabolic disorders including type 2 diabetes. Brown adipose tissue, which is specialized for energy expenditure via thermogenesis, has recently emerged as a potential therapeutic target for treating human obesity. Dr. Chang currently works on the molecular mechanisms of brwon fat thermogenesis with focus on understanding the roles of NT-PGC-1a, a short isoform of PGC-1a transcriptional coactivator.
SELECTED PUBLICATIONSPark JH, Kang HJ, Lee YK, Kang H, Kim J, Chung JH, Chang JS, McPherron AC, Lee SB. (2015). Inactivation of EWS reduces PGC-1a protein stability and mitochondrial homeostasis. PNAS. 112(19):6074-6079.
Henagan TM, Cefalu WT, Ribnicky DM, Noland RC, Dunville K, Campbell WW, Stewart LK, Forney LA, Gettys TW, Chang JS, Morrison CD. (2015). In vivo effects of dietary quercetin and quercetin-rich red onion extract on skeletal muscle mitochondria, metabolism, and insulin sensitivity. Genes & Nutrition. 10(1):451.
Jun HJ, Joshi Y, Patil Y, Noland RC, Chang JS. (2014). NT-PGC-1a activation attenuates high-fat diet-induced obesity by enhancing brown fat thermogenesis and adipose tissue oxidative metabolism. Diabetes, 63(11):3615-3625.
Wen X, Wu J, Chang JS, Wang J, Zhang Y, Gettys TW, Zhang Y (2014). Effect of exercise intensity on isoform-specific expressions of NT-PGC-1a mRNA in mouse skeletal muscle. BioMed Research International, 2014:402175.
Jun HJ, Gettys TW, Chang JS. (2012). Transcriptional activity of PGC-1a and NT-PGC-1a is differentially regulated by Twist-1 in brown fat metabolism. PPAR Res, 2012:320454.
Chang JS, Gettys TW. (2013). Analyzing phosphorylation-dependent regulation of subcellular localization and transcriptional activity of transcriptional coactivator NT-PGC-1alpha. Methods Mol Biol, 952:163-173.
Chang JS, Fernand V, Zhang Y, Shin J, Jun HJ, Joshi Y, Gettys TW. (2012). NT-PGC-1a is sufficient to link ß3-adrenergic receptor activation to the transcriptional and physiological components of adaptive thermogenesis. J Biol Chem, 287(12):9100-9111.
Chi, R.T., Torres, O.T., Segarra, V.A., Lansley, T., Chang, J.S., Newpher, T.M., Lemmon, S.K. (2012). Role of Scd5, a protein phosphatase-1 targeting protein, in phosphoregulation of Sla1 during endocytosis. Journal of Cell Science. 125:4728-4739.
Chang JS, Huypens P, Zhang Y, Black C, Kralli A, Gettys TW. (2010). Regulation of NT-PGC-1a subcellular localization and function of PKA-dependent modulation of nuclear export by CRM1. J Biol Chem, 285(23), 18039-18050.
Zhang Y, Huypens P, Adamson AW, Chang JS, Henagan TM, Boudreau A, Lenard NR, Burk D, Klein J, Perwitz N, Shin J, Fasshauer M, Kralli A, Gettys TW. (2009). Alternative mRNA splicing produces a novel biologically active short isoform of PGC-1 alpha. J Biol Chem, 284(47), 32813-32826.
Eiring AM, Neviani P, Santhanam R, Oaks JJ, Chang JS, Notari M, Perrotti D. (2008). Identification of novel post-transcriptional targets of the BCR/ABL oncoprotein by ribonomics: requirement of E2F3 for BCR/ABL leukemogenesis. Blood. 111(2):816-828.
Koschmieder S, D´Alo F, Radomska H, Schöneich C, Chang JS, Konopleva M, Perrotti D, Serve H, and Tenen DG. (2007). CDDO induces granulocytic differentiation of myeloid leukemic blasts through translational up-regulation of p42 CCAAT enhancer binding protein alpha. Blood. 110(10):3695-3705.
Chang JS, Santhanam R, Trotta R, Neviani P, Eiring AM, Briercheck E, Ronchetti M, Roy DC, Calabretta B, Caligiuri MA, and Perrotti D. (2007). High levels of the BCR/ABL oncoprotein are required for the MAPK-hnRNP E2-dependent suppression of C/EBPa-driven Myeloid Differentiation. Blood. 110(3):994-1003.
Notari M, Neviani P, Santhanam R, Blaser BW, Chang JS, Galietta A, Willis AE, Roy DC, Caligiuri MA, Marcucci G, Perrotti D. (2006). A MAPK/hnRNP K pathway controls BCR/ABL oncogenic potential by regulating c-myc mRNA translation. Blood. 107(6): 2507-2516.
Chang JS, Henry KR, Geli M, Lemmon SK. (2006). Cortical recruitment and nuclearcytoplasmic shuttling of Scd5p, a protein phosphatase-1 targeting protein involved in actin organization and endocytosis. Molecular Biology of the Cell. 17: 251–262.
Neviani P, Santhanam R, Trotta R, Notari N, Blaser BW, Liu S, Mao H, Chang JS, Galietta A, Caligiuri MA, Marcucci G, Perrotti D. (2005). The tumor suppressor PP2A is functionally inactivated in blast crisis CML through the inhibitory activity of the BCR/ABL-regulated SET protein. Cancer Cell. 8(5): 355–368.
Henry KR., D’Hondt K, Chang JS, Nix DA, Cope MJTV, Chan CSM, Drubin DG, Lemmon SK. (2003). The actin regulating kinase Prk1p negatively regulates Scd5p, a suppressor of clathrin deficiency, in actin organization and endocytosis. Current Biology. 13(17): 1564–1569.
Chang JS, Henry KR, Wolf BL, Geli M, Lemmon SK. (2002). Protein phosphatase-1 binding to Scd5p is important for regulation of actin organization and endocytosis in yeast. Journal of Biological Chemistry. 277(50): 48002–48008.
Henry KR, D’Hondt K, Chang JS, Newpher T, Huang K, Hudson RT, Riezman H, Lemmon SK. (2002). Scd5p and clathrin function are important for cortical actin organization, endocytosis, and localization of Sla2p in yeast. Molecular Biology of the Cell. 13(8): 2607–2625.
Chang JS, McPheeters DS. (2001). Identification of a U2/U6 helix Ia mutant that influences 3' splice site selection during nuclear pre-mRNA splicing. RNA. 6: 1120–1130. ^ top